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dc.contributor.author Brukhin, Vladimir B.
dc.contributor.author Jaciubek, Miloslawa
dc.contributor.author Carpio, Arturo Bolaños
dc.contributor.author Kuzmina, Vera
dc.contributor.author Grossniklaus, Ueli
dc.date.accessioned 2012-01-16T09:22:56Z
dc.date.available 2012-01-16T09:22:56Z
dc.date.issued 2012-01-16
dc.identifier.citation Brukhin , V B , Jaciubek , M , Carpio , A B , Kuzmina , V & Grossniklaus , U 2012 , ' Female gametophytic mutants of arabsidopsis thaliana identified in a gene trap insertional mutagenesis screen ' International Journal of Developmental Biology , vol 55 , no. 1 , pp. 73-84 . , 10.1387/ijdb.092989vb en
dc.identifier.issn 1696-3547
dc.identifier.other PURE: 174888
dc.identifier.other dspace: 2160/7737
dc.identifier.uri http://hdl.handle.net/2160/7737
dc.description Brukhin, V. B., Miloslawa, J., Carpio, A. B., Kuzmina, V., Grossniklaus, U. (2011). Female gametophytic mutants of arabsidopsis thaliana identified in a gene trap insertional mutagenesis screen. International Journal of Developmental Biology, 55, (1), 73-84. IMPF: 02.82 RONO: 00 en
dc.description.abstract In plants, the male and female gametophytes represent the haploid generation that alternates with the diploid sporophytic generation. Male and female gametophytes develop from haploid micro- and megaspores, respectively. In flowering plants (angiosperms), the spores themselves arise from the sporophyte through meiotic divisions of sporogenous cells in the reproductive organs of the flower. Male and female gametophytes contain two pairs of gametes that participate in double fertilization, a distinctive feature of angiosperms. In this paper, we describe the employment of a transposon-based gene trap system to identify mutations affecting the gametophytic phase of the plant life cycle. Mutants affecting female gametogenesis were identified in a two-step screen for (i) reduced fertility (seed abortion or undeveloped ovules) and (ii) segregation ratio distortion. Non-functional female gametophytes do not initiate seed development, leading to semi-sterility such that causal or linked alleles are transmitted at reduced frequency to the progeny (non-Mendelian segregation). From a population of 2,511 transposants, we identified 54 lines with reduced seed set (2%). Examination of their distorted segregation ratios and seed phenotypes led to the isolation of 12 gametophytic mutants, six of which are described herein. Chromosomal sequences flanking the transposon insertions were identified and physically mapped onto the genome sequence of Arabidopsis thaliana. Surprisingly, the insertion sites were often associated with chromosomal rearrangements, making it difficult to assign the mutant phenotypes to a specific gene. The mutants were classified according to the process affected at the time of arrest, i.e. showing mitotic, karyogamic, maternal or degenerative phenotypes. en
dc.format.extent 12 en
dc.language.iso eng
dc.relation.ispartof International Journal of Developmental Biology en
dc.title Female gametophytic mutants of arabsidopsis thaliana identified in a gene trap insertional mutagenesis screen en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1387/ijdb.092989vb
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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