Show simple item record Brukhin, Vladimir B. Jaciubek, Miloslawa Carpio, Arturo Bolaños Kuzmina, Vera Grossniklaus, Ueli 2012-01-16T09:22:56Z 2012-01-16T09:22:56Z 2012-01-16
dc.identifier.citation Brukhin , V B , Jaciubek , M , Carpio , A B , Kuzmina , V & Grossniklaus , U 2012 , ' Female gametophytic mutants of arabsidopsis thaliana identified in a gene trap insertional mutagenesis screen ' International Journal of Developmental Biology , vol 55 , no. 1 , pp. 73-84 . DOI: 10.1387/ijdb.092989vb en
dc.identifier.issn 1696-3547
dc.identifier.other PURE: 174888
dc.identifier.other PURE UUID: 0ce87baa-7b8a-4e3a-9703-6f8395714f30
dc.identifier.other dspace: 2160/7737
dc.identifier.other DSpace_20121128.csv: row: 4599
dc.identifier.other IBERS: 0000019138
dc.identifier.other Ibers_20121112_1204.csv: row: 1084
dc.identifier.other Scopus: 79952087116
dc.identifier.other PubMed: 21425082
dc.description Brukhin, V. B., Miloslawa, J., Carpio, A. B., Kuzmina, V., Grossniklaus, U. (2011). Female gametophytic mutants of arabsidopsis thaliana identified in a gene trap insertional mutagenesis screen. International Journal of Developmental Biology, 55, (1), 73-84. IMPF: 02.82 RONO: 00 en
dc.description.abstract In plants, the male and female gametophytes represent the haploid generation that alternates with the diploid sporophytic generation. Male and female gametophytes develop from haploid micro- and megaspores, respectively. In flowering plants (angiosperms), the spores themselves arise from the sporophyte through meiotic divisions of sporogenous cells in the reproductive organs of the flower. Male and female gametophytes contain two pairs of gametes that participate in double fertilization, a distinctive feature of angiosperms. In this paper, we describe the employment of a transposon-based gene trap system to identify mutations affecting the gametophytic phase of the plant life cycle. Mutants affecting female gametogenesis were identified in a two-step screen for (i) reduced fertility (seed abortion or undeveloped ovules) and (ii) segregation ratio distortion. Non-functional female gametophytes do not initiate seed development, leading to semi-sterility such that causal or linked alleles are transmitted at reduced frequency to the progeny (non-Mendelian segregation). From a population of 2,511 transposants, we identified 54 lines with reduced seed set (2%). Examination of their distorted segregation ratios and seed phenotypes led to the isolation of 12 gametophytic mutants, six of which are described herein. Chromosomal sequences flanking the transposon insertions were identified and physically mapped onto the genome sequence of Arabidopsis thaliana. Surprisingly, the insertion sites were often associated with chromosomal rearrangements, making it difficult to assign the mutant phenotypes to a specific gene. The mutants were classified according to the process affected at the time of arrest, i.e. showing mitotic, karyogamic, maternal or degenerative phenotypes. en
dc.format.extent 12 en
dc.language.iso eng
dc.relation.ispartof International Journal of Developmental Biology en
dc.rights en
dc.title Female gametophytic mutants of arabsidopsis thaliana identified in a gene trap insertional mutagenesis screen en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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