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dc.contributor.author Ahvenjärvi, Seppo
dc.contributor.author Vanhatalo, Aila
dc.contributor.author Shingfield, Kevin J
dc.contributor.author Huhtanen, Pekka
dc.date.accessioned 2013-11-14T22:02:28Z
dc.date.available 2013-11-14T22:02:28Z
dc.date.issued 2003-07
dc.identifier.citation Ahvenjärvi , S , Vanhatalo , A , Shingfield , K J & Huhtanen , P 2003 , ' Determination of digesta flow entering the omasal canal of dairy cows using different marker systems ' British Journal of Nutrition , vol 90 , no. 1 , pp. 41-52 . DOI: 10.1079/BJN2003876 en
dc.identifier.issn 0007-1145
dc.identifier.other PURE: 3536936
dc.identifier.other PURE UUID: 1cb3ff90-e763-43d3-9d42-67ccd79ce776
dc.identifier.other PubMed: 12844374
dc.identifier.other Scopus: 0042871383
dc.identifier.other handle.net: 2160/12583
dc.identifier.uri http://hdl.handle.net/2160/12583
dc.description.abstract Four studies were conducted to compare the effect of four indigestible markers (LiCoEDTA, Yb-acetate, Cr-mordanted straw and indigestible neutral-detergent fibre (INDF)) and three marker systems on the flow of digesta entering the omasal canal of lactating dairy cows. Samples of digesta aspirated from the omasal canal were pooled and separated using filtration and high-speed centrifugation into three fractions defined as the liquid phase, small particulate and large particulate matter. Co was primarily associated with the liquid phase, Yb was concentrated in small particulate matter, whilst Cr and INDF were associated with large particles. Digesta flow was calculated based on single markers or using the reconstitution system based on combinations of two (Co + Yb, Co + Cr and Co + INDF) or three markers (Co + Yb + Cr and Co + Yb + INDF). Use of single markers resulted in large differences between estimates of organic matter (OM) flow entering the omasal canal suggesting that samples were not representative of true digesta. Digesta appeared to consist of at least three phases that tended to separate during sampling. OM was concentrated in particulate matter, whilst the liquid phase consisted mainly of volatile fatty acids and inorganic matter. Yb was intimately associated with nitrogenous compounds, whereas Cr and INDF were concentrated in fibrous material. Current data indicated that marker systems based on Yb in combination with Cr or INDF are required for the accurate determination of OM, N and neutral-detergent fibre flow. In cases where the flow of water-soluble nutrients entering the omasal canal is also required, the marker system should also include Co. en
dc.format.extent 12 en
dc.language.iso eng
dc.relation.ispartof British Journal of Nutrition en
dc.rights en
dc.subject Animals en
dc.subject Biological Markers en
dc.subject Cattle en
dc.subject Chromium en
dc.subject Dietary Fiber en
dc.subject Digestion en
dc.subject Edetic Acid en
dc.subject Female en
dc.subject Gastrointestinal Contents en
dc.subject Gastrointestinal Transit en
dc.subject Lactation en
dc.subject Omasum en
dc.subject Ytterbium en
dc.title Determination of digesta flow entering the omasal canal of dairy cows using different marker systems en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.identifier.doi https://doi.org/10.1079/BJN2003876
dc.contributor.institution Department of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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