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dc.contributor.author Paillard, D.
dc.contributor.author McKain, N.
dc.contributor.author Rincon, M. T.
dc.contributor.author Shingfield, K. J.
dc.contributor.author Givens, D. I.
dc.contributor.author Wallace, R. J.
dc.date.accessioned 2013-11-18T21:10:19Z
dc.date.available 2013-11-18T21:10:19Z
dc.date.issued 2007-10
dc.identifier.citation Paillard , D , McKain , N , Rincon , M T , Shingfield , K J , Givens , D I & Wallace , R J 2007 , ' Quantification of ruminal Clostridium proteoclasticum by real-time PCR using a molecular beacon approach ' Journal of Applied Microbiology , vol 103 , no. 4 , pp. 1251-1261 . DOI: 10.1111/j.1365-2672.2007.03349.x en
dc.identifier.issn 1364-5072
dc.identifier.other PURE: 3538327
dc.identifier.other PURE UUID: 3ea2b1a8-6ec5-4c13-a8d0-5e17068eab34
dc.identifier.other PubMed: 17897229
dc.identifier.other Scopus: 34848849004
dc.identifier.other handle.net: 2160/12659
dc.identifier.uri http://hdl.handle.net/2160/12659
dc.description.abstract All members of the ruminal Butyrivibrio group convert linoleic acid (cis-9,cis-12-18:2) via conjugated 18:2 metabolites (mainly cis-9,trans-11-18:2, conjugated linoleic acid) to vaccenic acid (trans-11-18:1), but only members of a small branch, which includes Clostridium proteoclasticum, of this heterogeneous group further reduce vaccenic acid to stearic acid (18:0, SA). The aims of this study were to develop a real-time polymerase chain reaction (PCR) assay that would detect and quantify these key SA producers and to use this method to detect diet-associated changes in their populations in ruminal digesta of lactating cows. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Journal of Applied Microbiology en
dc.rights en
dc.subject Animal Nutritional Physiological Phenomena en
dc.subject Animals en
dc.subject Bacterial Typing Techniques en
dc.subject Butyrivibrio en
dc.subject Cattle en
dc.subject Clostridium en
dc.subject DNA, Bacterial en
dc.subject Diet en
dc.subject Female en
dc.subject Gastrointestinal Contents en
dc.subject Polymerase Chain Reaction en
dc.subject RNA, Bacterial en
dc.subject RNA, Ribosomal, 16S en
dc.subject Rumen en
dc.subject Stearic Acids en
dc.title Quantification of ruminal Clostridium proteoclasticum by real-time PCR using a molecular beacon approach en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.identifier.doi https://doi.org/10.1111/j.1365-2672.2007.03349.x
dc.contributor.institution Department of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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