Show simple item record Wilkinson, Toby Cowan, Alexander Vallin, Hannah Onime, L. A. Oyama, Linda Boniface Cameron, Simon Gonot, Charlotte Moorby, Jonathan Waddams, Kate Theobald, Vincent Leemans, David Bowra, S. Nixey, C. Huws, Sharon 2017-06-27T11:15:13Z 2017-06-27T11:15:13Z 2017-06-22
dc.identifier.citation Wilkinson , T , Cowan , A , Vallin , H , Onime , L A , Oyama , L B , Cameron , S , Gonot , C , Moorby , J , Waddams , K , Theobald , V , Leemans , D , Bowra , S , Nixey , C & Huws , S 2017 , ' Characterisation of the microbiome along the gastrointestinal tract of growing turkeys ' Frontiers in Microbiology , vol. 8 , 1089 . en
dc.identifier.issn 1664-302X
dc.identifier.other PURE: 17826585
dc.identifier.other PURE UUID: 4d237a16-9cf9-45c1-aff4-5e8610f1c71b
dc.identifier.other Scopus: 85021252367
dc.identifier.other PubMed: 28690591
dc.identifier.other PubMedCentral: PMC5479886
dc.identifier.other 2160/45307
dc.identifier.other ORCID: /0000-0002-4449-8432/work/47373958
dc.description.abstract The turkey microbiome is largely understudied, despite its relationship with bird health and growth, and the prevalence of human pathogens such as Campylobacter spp. In this study we investigated the microbiome within the small intestine (SI), caeca (C), large intestine (LI) and cloaca (CL) of turkeys at 6, 10 and 16 weeks of age. Eight turkeys were dissected within each age category and the contents of the SI, C, LI and CL were harvested. 16S rDNA based QPCR was performed on all samples and samples for the 4 locations within 3 birds/age group were sequenced using ion torrent-based sequencing of the 16S rDNA. Sequencing data showed on a genus level, an abundance of Lactobacillus, Streptococcus and Clostridium XI (38.2, 28.1 and 13.0% respectively) irrespective of location and age. The caeca exhibited the greatest microbiome diversity throughout the development of the turkey. PICRUSt data predicted an array of bacterial function, with most differences being apparent in the caeca of the turkeys as they matured. QPCR revealed that the caeca within 10 week old birds, contained the most Campylobacter spp. Understanding the microbial ecology of the turkey gastrointestinal tract is essential in terms of understanding production efficiency and in order to develop novel strategies for targeting Campylobacter spp en
dc.language.iso eng
dc.relation.ispartof Frontiers in Microbiology en
dc.rights en
dc.subject turkey en
dc.subject 16S rDNA en
dc.subject microbiome en
dc.subject Campylobacter en
dc.subject gastrointestinal tract en
dc.subject small intestine en
dc.subject casecum en
dc.subject large intestine en
dc.title Characterisation of the microbiome along the gastrointestinal tract of growing turkeys en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.description.version publishersversion en
dc.contributor.institution Department of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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