Show simple item record Leveau, Aymeric Reed, James Qiao, Xue Stephenson, Michael Mugford, Sam T. Melton, Rachel Rant, Jenni C. Vickerstaff, Robert Langdon, Timothy Osbourn, Anne 2018-11-06T20:00:10Z 2018-11-06T20:00:10Z 2019-02-01
dc.identifier.citation Leveau , A , Reed , J , Qiao , X , Stephenson , M , Mugford , S T , Melton , R , Rant , J C , Vickerstaff , R , Langdon , T & Osbourn , A 2019 , ' Towards take-all control: a C-‐21β oxidase required for acylation of triterpene defence compounds in oat ' New Phytologist , vol. 221 , no. 3 , pp. 1544-1555 . en
dc.identifier.issn 0028-646X
dc.identifier.other PURE: 28063976
dc.identifier.other PURE UUID: 710cbc9a-d0b0-4e76-b3d8-513e4fa84379
dc.identifier.other PubMed: 30294977
dc.identifier.other Scopus: 85054501122
dc.identifier.other 2160/47055
dc.identifier.other PubMedCentral: PMC6446040
dc.description.abstract Oats produce avenacins, antifungal triterpenes that are synthesized in the roots and provide protection against take‐all and other soilborne diseases. Avenacins are acylated at the carbon‐21 position of the triterpene scaffold, a modification critical for antifungal activity. We have previously characterized several steps in the avenacin pathway, including those required for acylation. However, transfer of the acyl group to the scaffold requires the C‐21β position to be oxidized first, by an as yet uncharacterized enzyme. We mined oat transcriptome data to identify candidate cytochrome P450 enzymes that may catalyse C‐21β oxidation. Candidates were screened for activity by transient expression in Nicotiana benthamiana. We identified a cytochrome P450 enzyme AsCYP72A475 as a triterpene C‐21β hydroxylase, and showed that expression of this enzyme together with early pathway steps yields C‐21β oxidized avenacin intermediates. We further demonstrate that AsCYP72A475 is synonymous with Sad6, a previously uncharacterized locus required for avenacin biosynthesis. sad6 mutants are compromised in avenacin acylation and have enhanced disease susceptibility. The discovery of AsCYP72A475 represents an important advance in the understanding of triterpene biosynthesis and paves the way for engineering the avenacin pathway into wheat and other cereals for control of take‐all and other diseases. en
dc.format.extent 12 en
dc.language.iso eng
dc.relation.ispartof New Phytologist en
dc.rights en
dc.subject Avena strigosa en
dc.subject avenacins en
dc.subject cytochromes P450 en
dc.subject disease resistance en
dc.subject metabolic engineering en
dc.subject natural products en
dc.subject triterpenes en
dc.title Towards take-all control: : a C-‐21β oxidase required for acylation of triterpene defence compounds in oat en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.description.version publishersversion en
dc.contributor.institution Department of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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