Show simple item record Traas, Jan A. Doonan, John H. Rawlins, David J. Shaw, Peter J. Watts, John Lloyd, Clive W. 2013-07-13T04:09:27Z 2013-07-13T04:09:27Z 1987-07-01
dc.identifier.citation Traas , J A , Doonan , J H , Rawlins , D J , Shaw , P J , Watts , J & Lloyd , C W 1987 , ' An actin network is present in the cytoplasm throughout the cell cycle of carrot cells and associates with the dividing nucleus ' Journal of Cell Biology , vol 105 , no. 1 , pp. 387-95 . DOI: 10.1083/jcb.105.1.387 en
dc.identifier.issn 0021-9525
dc.identifier.other PURE: 844743
dc.identifier.other PURE UUID: 03f81c93-a6e1-4137-8600-57fdc45150ae
dc.identifier.other PubMed: 2440896
dc.identifier.other Scopus: 0023368658
dc.identifier.other 2160/9069
dc.description.abstract We have studied the F-actin network in cycling suspension culture cells of carrot (Daucus carota L.) using rhodaminyl lysine phallotoxin (RLP). In addition to conventional fixation with formaldehyde, we have used two different nonfixation methods before adding RLP: extracting cells in a stabilizing buffer; inducing transient pores in the plasma membrane with pulses of direct current (electroporation). These alternative methods for introducing RLP revealed additional features of the actin network not seen in aldehyde-fixed cells. The three-dimensional organization of this network in nonflattened cells was demonstrated by projecting stereopairs derived from through-focal series of computer-enhanced images. F-actin is present in interphase cells in four interconnected configurations: a meshwork surrounding the nucleus; thick cables in transvacuolar strands and deep in the cytoplasm; a finer network of bundles within the cortical cytoplasm; even finer filaments that run in ordered transverse array around the cell periphery. The actin network is organized differently during division but it does not disappear as do the cortical microtubules. RLP stains a central filamentous cortical band as the chromatin begins to condense (preprophase); it stains the mitotic spindle (as recently shown by Seagull et al. [Seagull, R. W., M. Falconer, and C. A. Weerdenburg, 1987, J. Cell Biol., 104:995-1004] for aldehyde fixed suspension cells) and the cytokinetic apparatus (as shown by Clayton, L., and C. W. Lloyd, 1985, Exp. Cell Res., 156:231-238). However, it is now shown that an additional network of F-actin persists in the cytoplasm throughout division associating in turn with the preprophase band, the mitotic spindle, and the cytokinetic phragmoplast. en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof Journal of Cell Biology en
dc.rights en
dc.title An actin network is present in the cytoplasm throughout the cell cycle of carrot cells and associates with the dividing nucleus en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Department of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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